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Diagnostic Services Provided
Actinobacillus pleuropneumoniae
Serology
HNT – Hemolysin Neutralization Test
ELISA – Serotypes 1, 3, 5, 7
Serotype identification
PCR Based Strain Analysis
Actinobacillus
pleuropneumoniae
Bordetella bronchiseptic
Hemophilus parasuis
Moraxella bovis |
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PCR Based Identification
Actinobacillus
pleuropneumoniae
Bordetella bronchiseptica
Hemophilus parasuis
Moraxella bovis
Strep. canis - Under development
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APP Serology
Serologic evaluation of herds for
Actinobacillus pleuropneumoniae (App) has
proven to be a valuable tool in the prevention and control of porcine
pleuropneumonia and, in conjunction with clinical and pathologic
evaluations, has become the cornerstone of many quarantine and SEW
programs. It is important to remember that these serologic tests are
designed to be herd (population) tests and are not reliable when applied
to individual animals.
APP Hemolysin Neutralization (HNT) Assay
The HNT assay detects neutralizing antibodies produced by exposure Type
1 hemolysin/cytotoxin (Apx1) produced by some serotypes of App. The
assay is useful in the diagnosis of infection by App serotypes 1, 5, 9,
10 and 11. Currently available commercial and autogenous vaccines do not
induce HN titers.
APP ELISA
ELISA detects antibodies capable of binding capsular carbohydrates of
the reference strains of App. The available assays are useful in the
diagnosis of exposure to serotypes 1, 3, 5, and 7. However, pigs
infected with strains of serotype 1, 3, 5 or 7 that produce capsules
immunologically different from the type strain cannot be detected.
PCR
Diagnosis of infections by
organism specific Polymerase Chain Reaction (PCR) has become a widely
accepted and valuable tool, particularly in the diagnosis of organisms
that are difficult to grow in culture due to relatively slow growth or
special nutritional requirements.
Bordetella bronchiseptica and
Hemophilus parasuis
are cases in point. Additionally, using PCR it is possible to
distinguish closely related bacterial species and, in some cases, to
distinguish strains within a bacterial species.
We are currently offering PCR
based identification of:
Actinobacillus
pleuropneumoniae
Bordetella bronchiseptica
Hemophilus parasuis
Moraxella bovis
A
Streptococcus canis
assay is in developmental stage. We welcome submission of
Beta hemolytic Strep isolates for use in our research.
Important Sample
Submission Instructions
Due to stricter security on transport of bacterial samples,
transportation permits are now required. Prior to shipment of any
bacterial culture, please call 785-532-4421 to request a transportation
permit. All samples must be accompanied by proper documentation and a
current transportation permit.
Contact Information
Selected
Diagnostic Services
Phone: 785-532-4421
Fax: 785-532-4877
E-mail:
SelectedDiagnostics@vet.k-state.edu |
