Information for Submitting Samples for Swine Influenza Testing

Select Pigs by Symptoms: High fever, serous nasal discharge

Nasal swabs: Synthetic fiber swabs in transport media (such as BBL Culture swab with Stuarts media) or could be sent on 1 mL saline in snap cap tubes

Lung lavage fluid: Saline in leak-proof sterile containers (snap caps, blood tubes)

Fresh lung tissue: Affected portions of lung tissue, usually the hilar area and adjacent portions of cranial, middle and caudal lung lobes or ventral portions of these lobes; in whirlpacks, chilled not frozen, on ice packs.

Both PCR and Virus Isolation can be applied to these samples for SIV diagnosis.

Formalin-fixed lung tissue: Slices (1/2”) through affected portions of lung in 10% buffered formalin.

Follow routine diagnostic sample packaging guidelines: Double bag specimens or line shipping container with plastic bag, preferably in Styrofoam-lined container with coolant packs.

The samples described above would also be useful for diagnostic of any acute respiratory disease in swine.

Diagnostic Note: Dr. Richt’s research group has recently developed differential PCR assay that will distinguish the swine-like human variant from commonly circulating SIV strains.

Pooling: Swabs can be pooled in groups of 5 for PCR testing.

Shipping Address:
KANSAS STATE VETERINARY DIAGNOSTIC LABORATORY
Mosier D-117, 1800 Denison Avenue
Manhattan, KS 66506-5601

Sample selection and Fee Schedule: go to Fee/Test Schedule

Submission form: Download a KSVDL submission form

Contact Information:
Phone (785)-532-5650; Toll Free (866)-512-5650; Fax (785) 532-4481; Web www.ksvdl.org