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The Flow Cytometry laboratory is housed in Coles
Hall Room 324 of the College of Veterinary Medicine Complex. This laboratory is
equipped with state of the art equipment by Becton Dickinson, a
FACSCalibur and FACSVantage SE.
The FACSCalibur , is a bench top flow cytometer equipped with two
lasers, an argon and Red diode laser, with emission at 488 and 630 nm.
This instrument has the capability for 4-color analysis plus forward and
side scatter. |
The FACSVantage SE (photo), is an analytical flow cytometer with sorting
capabilities. This instrument has two lasers with three distinct lines
of emission. The Enterprise II laser has an argon and UV line, whereas
the Helium Neon laser emits at 630 nm. This instrument has 6-color
capabilities and can sort 7,000 cells/second. |
The flow cytometers are interfaced with G4 Macintosh computers and
multiple printers that have publication quality color capabilities.
Analysis software for routine analysis is Cell QuestTM and for DNA
analysis we have ModFitTM available.
Research experiments that can be done with this equipment are limited
only by your imagination. The following is a representation of the types
of samples and assays that have been performed in this laboratory by
various investigators (See slide show:
Basic
Theories and Applications for Flow Cytometry) (Requires Adobe
Acrobat Reader).
- Characterize molecule expression on cells using
fluorochrome conjugated antibodies or immunophenotyping subsets of
immune cells
- Apoptosis studies
- Determine DNA content and viability of cells
including spermatozoa
- Determine intracellular calcium levels
- Assess respiratory burst and phagocytic
function in neutrophils
- Label bacteria
- Determine DNA cell cycle phases in cells (G0,
G1, S, G2 + M)
- Determine enzyme activity of cells using
fluorochrome conjugated substrates
- Sort rare cells or GFP expressing cells
Publications Generated Using the Flow Cytometry
Facility at KSU
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