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Kansas State University

Faculty

 

Raymond Rowland

 

 

Raymond R. R. Rowland
PhD
Associate Professor
L-229 Mosier Hall
Phone: 785-532-4631
Fax: 785-532-4481
Email browland@vet.k-state.edu

 

 

 

Research

The overall goal of my research is to investigate emerging disease problems in swine, with an emphasis on viral infections. The bulk of these efforts are directed at elucidating molecular mechanisms of viral persistence and virulence caused by porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus 2 (PCV2) considered the most important infectious disease problems of swine. In addition to their economic impact, both viruses represent relevant biological systems for understanding mechanisms of viral pathogenesis, including evasion strategies. Research focuses at the population, cellular and molecular levels and incorporates the use of reverse genetic systems. The results are being applied to the development of novel vaccine and diagnostic testing strategies.
 

Selected Publications

Horlen, K, P Schneider, J Anderson, JC Nietfeld, SC Henry, LB Tokach, and RRR Rowland. 2007. A cluster of farms experiencing severe porcine circovirus associated disease (PCVAD): Clinical features and association with the PCV2-321 genotype. Accepted for publication, J Swine Health and Prod.

Fang, Y, P Schneider, W Zhang, KS Faaberg, EA Nelson, and RRR Rowland. 2007. Diversity and evolution of a newly emerged North American Type 1 porcine arterivirus. Accepted for publication, Archiv. Virol.

Cafruny, WA, RG Duman, G Wong, S Suleman, P Ward-Demo, RRR Rowland and EA Nelson. 2006. Porcine reproductive and respiratory syndrome virus (PRRSV) infection spreads by cell-to-cell transfer in cultured MARC-145 cells, is dependent on an intact cytoskeleton, and is suppressed by drug-targeting of cell permissiveness to viral infection. In press, Virol. J.

Fang,Y, RRR Rowland, M Roof, JK Lunney, J Christopher-Hennings and EA Nelson. 2006. A Full-length cDNA Infectious Clone of North American Type 1 Porcine Reproductive and Respiratory Syndrome Virus: Expression of Green Fluorescent Protein in the Nsp2 Region. J Virol. In Press.

Christopher-Hennings J, M Dammen, E Nelson , RRR Rowland, and R Oberst. 2006. Comparison of RNA extraction methods for the detection of porcine reproductive and respiratory syndrome virus from boar semen. J Virol Meth 136:248-253.

Fang, Y, A Pekosz, L Haynes, EA Nelson, and RRR Rowland. 2006. Production and characterization of monoclonal antibodies against the nucleocapsid protein of Sars-CoV. Adv Exp Med Biol. In press

Plagemann, PGW, RRR Rowland and WA Cafruny. 2005. Polyclonal hypergammaglobulinemia and formation of hydrophobic immune complexes in porcine reproductive and respiratory syndrome virus-infected and uninfected Pigs. Viral Immunol 18: 138-147.

Wu, WH, Y Fang, RR Rowland, SR Lawson, J Christopher-Hennings, KJ Yoon and EA Nelson. 2005. The 2b protein as a minor structural component of PRRSV.Virus Res 114:177-181.

Rowland, RRR, V Chauhan, Y Fang, A Pekosz, M Kerrigan and MD Burton. 2005. Intracellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein: absence of nucleolar accumulation during infection and after expression as a recombinant protein in vero cells. J Virol 79:11507-11512.

Fang Y, DY Kim , S Ropp, P Steen, J Christopher-Hennings, EA Nelson EA and RRR Rowland. 2004 Heterogeneity in Nsp2 of European-like porcine reproductive and respiratory syndrome viruses isolated in the United States. Virus Res 100:229-235.

Rowland, RRR, S Lawson, K Rossow and DA Benfield 2003. Lymphotropism of porcine reproductive and respiratory syndrome virus replication during persistent infection of pigs originally exposed to virus in utero. Vet Micro 96: 219-235.