Faculty
Lisa C. Freeman
PhD, Pharmacology, The
Ohio State University, 1989
DVM, Cornell University, 1986
MS, Reproductive Physiology, Cornell University, 1986
BS, Biology, Cornell University, 1981
Phone: (785) 532-4542
Fax: (785) 532-4557
e-mail: freeman@vet.k-state.edu
Plaque text:
Association For Women Veterinarians
2002 Outstanding Woman Veterinarian of The Year
Presented to Dr. Lisa Freeman
For Professional Excellence and Achievement in the Field of
Veterinary Medicine
Research Interests:
Dr. Freeman's laboratory is interested in the basis for tissue-specific differences in ion channel signaling. The present focus is determining the functional significance of potassium, calcium and chloride channels in non-nerve, non-muscle cells, particularly ovarian and intestinal epithelial cells. The knowledge obtained will increase understanding and enable better treatment of ovarian cancer and intestinal ulcers.
The Freeman lab uses patch-clamp techniques to record ionic currents from isolated cells. The biophysical and pharmacological properties of the currents lead to hypothesis about the currents' molecular determinants and functional significance. Hypothesis testing is accomplished by complimenting patch-clamp recording with microarray analysis, RT-PCR, SDS-PAGE, cell culture, flow cytometry, confocal microscopy and heterologous expression of ion channel proteins.
Dr. Freeman is also interested in enhancing the participation of veterinarians in comparative biomedical research. She contributes actively to several funded research projects that support this objective.
Selected Publications:
Freeman LC, Lippold JJ, Mitchell KE (2000) Glycosylation affects gating and pH sensitivity of IsK channels. J Membrane Biology 177:65-79.
Mason DE, Mitchell KE, Li Y, Finley M,Freeman LC. (2002) Molecular basis of voltage-dependent potassium channels in porcine granulosa cells. Mol Pharm 61:201-213.
Finley MR, Li Y, Hua F, Lillich J, Mitchell KE, Gilmour, RF, Freeman LC. (2002) Expression and co-association of ERG1, KCNQ1 and KCNE1 potassium channel proteins in horse heart. Am J Physiol 283: H126-138.
Manikkam M, Li Y, Mitchell BM, Mason DE, Freeman LC. (2002) K+ channel antagonists influence granulosa cell proliferation, differentiation and apoptosis. Biol Reprod 67:88-98.
Lillich JD, Rakestrasw PC, Roussel AJ, Finley MR, Ganta S, Freeman LC. (2003)The Ether-a-go-go (ERG) potassium channel is expressed in equine GI smooth muscle and influences jejunal smooth muscle activity. American Journal of Veterinary Research 64:267-272.
Li Y, Ganta S, von Stein FB, Mason DE, Mitchell BM, Freeman LC. (2003) 4-aminopyridine Decreases Progesterone Production by Porcine Granulosa Cells. Reproductive Biology & Endocrinology 1:31.
Finley MR, Lillich JD, Gilmour RF, Freeman LC. (2003) Structural and Functional Bases for the Long QT Syndrome: Relevance to Veterinary Patients. J Vet Int Med 17:473-488.
Arnold S, Dyer RA, Freeman LC, Montelone B, Spears JD. (2004) The GROW Project: ”The Road Ahead”. 2004 WEPAN (Women In Engineering Programs and Advocates Network) National Conference Proceedings.
Freeman LC (2005) Rx for Recruitment and Retention of Veterinarian
Scientists: Money, Marketing, Mentoring. J Veterinary Medical Education
(in press)